WP3-T1 Definition of criteria and development of methods for pathogen survival evaluation

Participants :

  • 1 - IFREMER
  • 4 - CVI-IMARES
  • 5 - IRTA
  • 7 - UNIGE

Criteria and methods to measure pathogen survival will be defined and developed in order to establish the viability (i.e. live/dead) status and/or pathogenicity potential and/or infective/non-infective status of pathogens including OsHV-1, Vibrio splendidus, V. aestuarianus, Nocardia crassostreae and Marteilia refringens.

For bacteria belonging to Vibrio genus, criteria and methods will include viability assays, assessment of viable but non-culturable state (VBNC), analysis of adhesive properties and capability to form biofilms on biotic and abiotic substrates, persistence in sediments and bulk water, evaluation of virulence-related traits (link with WP4), and transmission/exposure trials.

In addition to screening (real time PCR) and culture of Nocardia crassostreae from sediment (WP2 - T2), laboratory tests will be carried out to assess the survival of N. crassostreae in sediment and seawater.

In addition to OsHV-1 and Marteila refringens screening by real time PCR from sediment, water, zooplakton and macro fauna samples (WP2 - T2), laboratory tests will be carried out to assess the pathogenicity of both pathogens found in the different environmental compartments through experimental infection assays.

The criteria and methods defined during the course of this task (WP3 - T1) will then be used in Task 2 of this workpackage.