WP4-T1 Development of experimental disease models

Participants :

  • 1 - IFREMER
  • 2 - CSIC
  • 11 - ATLANTIUM

Experimental disease (bacterial and viral) models will be developed and used in order to explore the susceptibility of mussels and Pacific oysters to selected pathogens including OsHV-1 and Vibrio species and to study pathogen/host interactions in controlled conditions. Experimental disease models will provide contrasted biological material in terms of disease susceptibility such as infected versus non-infected animals, susceptible versus non-susceptible animals of the same species (families displaying different susceptibilities to mortality/infection with OsHV-1 and Vibrio species) and resistant versus susceptible species (mussels versus oysters) for the following tasks (WP4, Tasks 2 and 3).

Several infection protocols will be used such as injection and cohabitation in order to determine the effect of pathogens on bivalve mortalities but also on the host immune response and on the expression of virulence factors. The techniques that will be used have been already described (Gagnaire et al., 2007; Gay et al., 2004, Schikorski et al., in press). Samples will be collected, distributed and used for the following tasks.

Biohazardous material used in laboratory assays will be autoclaved at 121 degrees for 30 minutes. Shellfish waste will be placed in a bin lined with an absorbent pad bottom and top, and sealed with a lid. The bins will be removed by an approved contractor for waste disposal and taken for incineration.

As UV (HOD power) inactivates the pathogens by causing damage to the DNA or RNA, the capacity of DNA repair of OsHV-1 and Vibrio species will be explored. The short-term and the long-term effects of the UV inactivated pathogens on bivalves will thus be assessed through experimental trials. Bivalves will be subjected to pathogens (OsHV-1 and Vibrio species) that were exposed to the HOD germicidal power by using a static UV setup -Collimated Beam Apparatus (CBA) -.based on monochromatic (Low Pressure UV lamps) UV light and polychromatic (Medium Pressure UV Lamps).

Participant 11 will manufacture and supply the UV devices (CBA devices) to Participants 1 and 2 and will prepare the protocols for UV illumination tests on the target pathogens for evaluating. Participant 11 will participate at part of the tests and will train the local researchers to operate the CBA devices. In addition Atlantium's researchers will participate in analysing the results and preparing the reports for generating the data for other WP's mainly WP7